Eve's Apples are an addictive, dangerous extension of The Tree of Life, growing exclusively near the top of the tree. They are a melon-sized fruit shaped like an apple that is a deep, crimson-red in colour. While the taste and texture, alongside the juiciness are decadent, the adverse effects of the fruit are gruesome and ruthless. Even before having a bite of the fruit, anyone who encounters it will feel a strange compulsion towards it, but will face dire consequences due to its addictive nature, hence its name. No matter how much of this fruit one eats, they will never be satiated and will never feel as though their stomach is being filled — the fruit has a numbing effect on the inside of one's stomach, tragically leading many to eat until their stomach bursts and succumb to internal bleeding. It is a horrible way to go out, so all advice orders avoiding the fruit.

Naturally growing exclusively amongst the roots of the tree, waterberries are a near-tasteless, beige-ish clear berry composed almost entirely of water. They are equipped with a concotion of essential vitamins and minerals, leading many to plant and harvest them on other levels, as well as up the length of the tree.

While not the most popular option, dewnuts are a vital source of nourishment if one is in a pinch. Their slimy, brown, dew-like exterior is less than appealing, but upon cracking the shell, dewnuts posses a similar texture, taste, and nutritional profile to cashews, only denser and more filling presumably from the amount of sunlight and nutrients they are provided from the conditions of the level. They can be found just about anywhere growing from The Tree of Life.

Found only near the highest regions of the level, blue raspberries are perhaps the most valuable resource it has to offer. To their natural counterparts, moss bears, they provide the ability to grow and spread moss and shrubbery wherever they go, but when consumed by other living beings, they give one excessive stamina and regenerative abilities. This effect is only possible via a cocktail of natural hormone boosters and stimulants that still are not fully understood. Regardless, they are only able to grow in high altitudes and in very particular conditions, making harvesting them unfortunately next to impossible with today's advancements. Their taste has been described as a sour, powerful punch akin to the signature manufactured "blue raspberry" taste of many candies from the frontrooms.

Aside from the few species that are exclusive to the level, there is a wide variety of different fruits, trees, and plants that grow in different areas of the level. A consistent factor, however, is that each branch houses similar types of flora and fauna. For example, one could expect to find pine trees and cranberry bushes growing on the same branch as common wolves and some species of bears. The biodiversity found on The Tree of Life is one of a kind; the ecosystem on the level is so complex that it is difficult to understand. Some branches even host deserts — as the tree people have explained it, drafts in the wind created by 'guiding spirits' make phenomena such as this possible. Regardless, it is a miraculous feat of life to be admired.

Existing perhaps only in fiction, the fruit of eternal life is said to be found at the height of the tree, told to be dragonfruit-esque in appearance with a turquoise hue. Some say they have heard true stories passed down through generations of its discovery and the tales of its consumers, but it seems as though none are alive today to tell their own tale.

Better known as tree people, this sentient species has been around since the beginning of time in the Backrooms. As such, they are wise beyond their many years, many acting as spiritual leaders to the human locals. Their biology is quite simple — they have two arms and two legs, similar in composition to the tree trunks of sequoia trees. Their fingers, toes, and other appendages are more akin to the branches. They vary in size in a similar way that humans do, though they lack variety in their thickness and frame. They do not have digestive and reproductive organs, as is the norm for plants, however they also do not produce seeds. They are conceived simply by emerging from the tree's lower branches; at birth, they could be mistaken for twigs, as they gradually sprout from the branches before gaining sentience.

With their lack of a digestive track, tree people also lack vocal chords as well as any oral or nasal orifice, though they do possess three black, bead-like eyes in a triangular arrangement on their face. They seem to be unable to feel pain due to their lack of nerve endings, and have no way of hearing either, making sight their only conventional sense. They have attempted to explain, however, that they do feel another kind of sensation that relates to other plantlife — a sixth sense of sorts that allows them to communicate with one another, as well as other plants, The Tree of Life included.

Socially, tree people are quite different from humans as could be expected. They do not have childhood or adolescent stages of development as most species do, nor do they have typical family dynamics. Rather, they seem themselves as one joint family, other species of plants being their brothers, sisters, and cousins and The Tree of Life their parent.

Contrary to their kindred appearances, daybats are much different from their standard counterparts. They are a much larger, heartier species that average at about 8 feet tall, possessing a wingspan of around 12 feet. Their wings are much more thick and leathery, whilst still being quite light, allowing for them to fly quite comfortably and hover even when carrying a human being.

Daybats' looks can be quite deceiving — they are often described as frightening, hideous creatures, but in reality are quite valuable companions. They act comparably to most domestic birds, easily building relationships with both humans and tree people. Their main source of food is dewnuts, leading them to inhabit groves up nearly the entire length of the tree. Their behavioral tendencies such as the way they perch, reproduce, and interact with each other are near-identical to that of bats, but their sensory capabilities are quite the opposite. Daybats are almost entirely deaf, relying heavily on their keen sense of sight to survive and using unique gestures with their wings to communicate rather than their usual method of echolocation.

Overall, daybats are a welcomed companion by all on and off the level, as some have become domesticated and joined wanderers on their journey, while others have been transported to other habitable levels and kept as pets.

Moss bears are a particular species, not much being known about them or why they act the way they do. They seem to be composed almost entirely of a moss-like coat of fur, though they do possess a small body at the centre of it. They are completely indifferent to both each other and anyone who attempts to interact with them. Their sole purpose, as it seems, is spreading moss using the effects of blue raspberries until it engulfs their entire surrounding area. After this, they become dormant, not eating, nor sleeping, nor exactly doing anything. As a precaution, most inhabitants of the level have agreed to keep their distance from these creatures, their almost ghost-like aura warranting subconscious caution.

As aforementioned, the biodiversity of The Tree of Life knows no bounds. All species of birds, insects, amphibians, reptiles, primates — all living creatures can be found here somewhere. One distinct feature, however, of some of the fauna of this level, is that they give off a strong glow. People have reporting spotting gleaming butterflies, squirrels, and hummingbirds, though no one has been able to capture them so all evidence is unconfirmed.

No one is quite sure what these beings are or whether they even exist or not, but "tree angels" is the designation used for the glowing figures seen in the distance as one nears the treetop. Many have speculated that they are assigned to guard whatever is at the top of The Tree of Life, while others have dismissed the theory due to how far-fetched it sounds. Recent rumours of different glowing animals and insects, however, have reignited the circulation of conspiracies around the level.

M.E.G. Base Rhizo is an instrumental hub for all botanical practices and the cultivation of a large sum of the M.E.G.'s food supplies. Due to the level's pristine conditions, several greenhouses and growth plots have been set up all around The Tree of Life's perimeter, alongside dozens of permanent residences of generational farmers. Several supply chains have been formed to deliver goods from the level to myriad other M.E.G. outposts and bases across the Backrooms, feeding hundreds if not thousands of people. As such, the M.E.G. invests quite a bit in the security of their harvest, boasting quite a bit of manpower in order to ward off other groups that have attempted to tamper with their system, such as Amor Incrementum.

The base is welcoming to newcomers, and is a great place to settle down, especially if one has been "on the run" so to speak for some time.

Located at the centre of Bough City, M.E.G. Outpost Thamnos is about as far up The Tree of Life that the group is willing to venture, mainly used as a hub for communication with the tree people and populated by their sharpest minds to learn about how to cultivate different types of plants native to the Backrooms.

Extending a large sector of the way up the lower branches, Bough City is the level's second largest settlement, coming somewhat close in numbers to Base Rhizo, though the population distribution leans much more in favour of tree people. Houses made of cobblestone are the main staple of the town, having been brought up the tree in a process that took the tree people's ancestors an unfathomable amount of time to complete — all because they refused to cut down any organic matter.

Communal buildings such as bathhouses and markets where goods are traded are sprinkled about the houses. With how the tree people consume water, bathing has become a ritual of sorts for them, where they relax and indulge. Human sections of these bathhouses are often separate from the rest, as well use them to clean ourselves, which would dirty they water they collect for drinking.

Life in Bough City is quite mundane, as tree people are a humble bunch who do not exactly seek to advance or escape.

A hostile bunch that began congregating near the top of The Tree of Life in the last dozen or so years, the Climbers' sole purpose is finding their way to the top. As they are out of the way of most people, they are largely left alone, though they do exhibit an alarming obsession with their goal, acting almost as if they know something that others do not.